Tarbiat Modares University Journal of Agricultural Science and Technology 1680-7073 12 2 2010 04 01 Natural Deoxynivalenol Contamination of Corn Produced in Golestan and Moqan Areas in Iran 233 239 15322 EN R. Karami-Osboo Mycotoxins Research Laboratory, Plant Pests and Diseases Research Institute, Tehran, Islamic Republic of Iran. M. Mirabolfathi Mycotoxins Research Laboratory, Plant Pests and Diseases Research Institute, Tehran, Islamic Republic of Iran. F. Aliakbari Islamic Azad University, Tehran, Islamic Republic of Iran. Journal Article Deoxynivalenol contamination was determined in corn produced in Golestan and Ardabil (Moqan) Provinces, Iran, in 2004-2005. Samples were collected from different stages of production, including before harvest, at harvest, post harvest as well as after drying. Ground sub samples were extracted with water; each extracted sample was cleaned up through an immunoaffinity column. Deoxynivalenol was estimated through reversed-phase High Performance Liquid Chromatography (HPLC). The linearity of standard curve for 50 -10,000 ng ml-1 of standard solutions was proved (R2= 0.9999). Detection limit was 10 ng g-1. Recovery of the method for 1,000 and 500 ng g-1 spiked samples was 73.5% and 93.5% (n= 5). Deoxynivalenol contamination was found in 76.7% of samples in the range of 54.4-518.4 ng g-1. The mean of contamination was 116.25 ng g-1. This is the first report of natural DON contamination of corn from Iran. https://jast.modares.ac.ir/article_15322_fe184ec3276a986afa6e10163a63a942.pdf