Tarbiat Modares University Journal of Agricultural Science and Technology 1680-7073 14 5 2012 09 01 Supercritical Fluid Extraction of Tobacco Seed Oil and its Comparison with Solvent Extraction Methods 1053 1065 15145 EN S. Majdi Department of Chemistry, K. N. Toosi University of Technology, Tehran, Iran. M. Barzegar Department of Food Science and Technology, Tarbiat Modares University, P. O. Box: 14115-336, Tehran, Islamic Republic of Iran. A. Jabbari Department of Chemistry, K. N. Toosi University of Technology, Tehran, Iran. M. AghaAlikhani Department of Agronomy, Tarbiat Modares University, Tehran, Islamic Republic of Iran. Journal Article Tobacco (<em>Nicotiana tabacum</em> L.) is an important industrial crop and its seeds contain significant amounts of oil. The extraction of oil components using solvent at high pressure, or supercritical fluid (SCF), has received much attention. In the present study, statistical analyses showed that the average extraction yield of seed oil of five tobacco varieties using SFE was 9.33%, which was higher than Sonication (7.75%) and DGF (Deutsche Gesellschaft f_r Fettwissenschaft) standard method B-I5(87) (8.48%), but lower than Soxhlet (13.72%). Also, fatty acids profile of each extracted oil was determined by gas chromatography. Various saturated and unsaturated fatty acids such as lauric (C12:0), myristic (C14:0), palmitic (C16:0), palmitoleic (C16:1), stearic (C18:0), oleic (C18:1), linoleic (C18:2), linolenic (C18:3) and eicosanoic (C20:0) acids were observed in the extracted oils. https://jast.modares.ac.ir/article_15145_c52dc29202c3434f6ba66017a4e5b676.pdf