Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1396
2
1
gregorian
2017
5
1
19
3
online
1
fulltext
en
Anticholinesterase Potential of Monoterpenoids on the Whitefly Bemisia tabaci and Their Kinetic Studies
B-biotype <em>Bemisia tabaci</em> is a severe insect pest worldwide in many ornamental, agricultural, and horticultural crops. Control of this insect is obstructed by resistance to many AcetylCholinEsterase (AChE)-inhibiting insecticides, such as organophosphates and carbamates. In the present work, we evaluated the acetylcholinesterase inhibitory activity of six monoterpenoids namely α-pinene, terpineol, linalool, ß-myrcene, nerol and geraniol<em> in vitro</em> and <em>in vivo</em>. Inhibition of AChE of <em>B. tabaci</em> was measured by colorimetric method. The results showed that all of the monoterpenoids produced AChE inhibitory activity, with <em>IC<sub>50</sub> </em>values ranging from 0.96 to 26.85 mM. Alpha-pinene showed the most potent inhibitory activity (IC<sub>50</sub>= 0.96 mM). Kinetic analysis showed reversible non-competitive type inhibition, revealing that these components might bind both the enzyme alone and the enzyme-substrate. Results demonstrate the AChE inhibitory activity as mode of action of these monoterpenoids at relatively high concentrations. Thus, this could be useful for investigation of new ecofriendly natural insecticidal compounds.
Acetylcholinesterase,Bemisia tabaci,IPM,Monoterpenoids,Non-competitive inhibition
643
652
http://jast.modares.ac.ir/browse.php?a_code=A-23-14989-1&slc_lang=en&sid=23
K.
Zarred
K.
Zarred
100319475328460068833
100319475328460068833
Yes
Institut Supérieur Agronomique of Chott Mariem (ISA), University of Sousse, Tunisia.
Institut Supérieur Agronomique of Chott Mariem (ISA), University of Sousse, Tunisia.
A.
Laarif
A.
Laarif
100319475328460056202
100319475328460056202
No
UR13AGR09, Regional Center for Research in Horticulture and Biological Agriculture (CRRHAB), University of Sousse, Tunisia.
UR13AGR09, Regional Center for Research in Horticulture and Biological Agriculture (CRRHAB), University of Sousse, Tunisia.
A.
Ben Hamouda
A.
Ben Hamouda
100319475328460056860
100319475328460056860
No
UR13AGR09, Regional Center for Research in Horticulture and Biological Agriculture (CRRHAB), University of Sousse, Tunisia.
UR13AGR09, Regional Center for Research in Horticulture and Biological Agriculture (CRRHAB), University of Sousse, Tunisia.
I.
Chaieb
I.
Chaieb
100319475328460056817
100319475328460056817
No
Laboratory of Plant Protection, National Institute of Agronomic Research of Tunisia (INRAT), University of Carthage, Tunisia.
Laboratory of Plant Protection, National Institute of Agronomic Research of Tunisia (INRAT), University of Carthage, Tunisia.
J.
Mediouni-Ben Jemaa
J.
Mediouni-Ben Jemaa
100319475328460056807
100319475328460056807
No
Laboratory of Biotechnology Applied to Agriculture, National Institute of Agronomic Research of Tunisia (INRAT), Tunisia.
Laboratory of Biotechnology Applied to Agriculture, National Institute of Agronomic Research of Tunisia (INRAT), Tunisia.