Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1392
8
1
gregorian
2013
11
1
15
6
online
1
fulltext
en
Presence of Multiple cry Genes in Bacillus thuringiensis Isolated from Dead Cotton Bollworm Heliothis armigera
<em>Cry</em> genes encoding Cry proteins toxic to Lepidoptera, Coleoptera and Diptera species were studied in thirty seven <em>B. thuringiensis </em>strains isolated from twelve naturally infested <em>Heliothis armigera</em> larvae. To further confirm the isolates, two groups of species-indicative biochemical tests were applied while discriminative biochemical tests being employed to figure out the repetitive strains. A PCR experiment was performed using five sets of universal primers for <em>cry1, cry2, cry3, cry4, cry7/8</em> genes. All strains reacted appropriately, for <em>B. thuringiensis</em>, to the biochemical tests and while the reactions to the discriminative tests being varied. Based upon the results of the discriminative tests, twenty four non-repetitive strains were selected and employed in the PCR assay. Each of the selected strains presented one <em>cry</em> gene, at least; <em>cry1</em> being the most frequently detected one (91.7%), followed by <em>cry2</em> (87.6%), <em>cry3</em> (50%) and <em>cry4</em> (42%) but no isolate harbored a coleopteran-active <em>cry7/8</em> gene. All the strains presented combinations of two or more <em>cry</em> genes: 20% presenting <em>cry1+cry2</em>, 12.5% <em>cry1+cry3</em>, 4% <em>cry2+cry4</em>, 20%<em> cry1+cry2+cry3</em>, 20% <em>cry1</em>+<em>cry2+cry4</em>, 4% <em>cry1+cry3+cry4</em> and 12.5% carrying all the four <em>cry</em> genes studied and only one strain bearing a single <em>cry</em> gene. The <em>cry1-cry2</em> combination was common in many strains (72.5%). Genetic characterization of this collection provides an opportunity for selection of strains with improved and multiple insecticidal toxicity.
Bacillus thuringiensis,Biochemical characters,Cry,PCR
1285
1295
http://jast.modares.ac.ir/browse.php?a_code=A-23-11598-113&slc_lang=en&sid=23
S.
Khojan
S.
Khojan
100319475328460046836
100319475328460046836
No
Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
M.
Keshavarzi
M.
Keshavarzi
100319475328460046835
100319475328460046835
Yes
Seed and Plant Improvement Institute, Karaj, Islamic Republic of Iran.
Seed and Plant Improvement Institute, Karaj, Islamic Republic of Iran.
K.
Zargari
K.
Zargari
100319475328460046837
100319475328460046837
No
Department of Agronomy, Varamin Branch, Islamic Azad University, Islamic Republic of Iran.
Department of Agronomy, Varamin Branch, Islamic Azad University, Islamic Republic of Iran.
H.
Abdolahi
H.
Abdolahi
100319475328460046838
100319475328460046838
No
Seed and Plant Improvement Institute, Karaj, Islamic Republic of Iran.
Seed and Plant Improvement Institute, Karaj, Islamic Republic of Iran.
F.
Rouzbeh
F.
Rouzbeh
100319475328460046839
100319475328460046839
No
Sugarbeet Seed Institute, Karaj, Islamic Republic of Iran.
Sugarbeet Seed Institute, Karaj, Islamic Republic of Iran.