Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1392
6
1
gregorian
2013
9
1
15
5
online
1
fulltext
en
Effect of Paternal Genotypes Sprays with BA and IAA Concentration on Embryo Rescue of F1 Progenies from 'Askari' (Vitis vinifera L.) Cultivar
The major objective of seedless grape breeding program is production of grapes with high quality. Breeding of seedless grapes is difficult because the embryos are aborted after fertilization. Embryo rescue technique is utilized to obtain plants from crosses between seedless cultivars. In the present study, the effect of paternal genotypes and different IAA concentrations in presence of BA applications were investigated on embryo rescue in Askari cultivar in randomized complete block design with three replications. Sprays with BA were performed two times at 18 E-L and 27 E-L stages. Hybrid embryos were produced from the cross between Askari cultivar as a female parent and Ruby Seedless, Bidane Sefid, and Bidane Ghermez as male parents. Forty days after pollination (at 29 E-L stage), ovules were cultured on NN medium with three different concentrations of IAA (1, 1.75, 3 mg L<sup>-1</sup>). The results showed that male genotypes and different concentrations of IAA on the media had a significant effect on embryo germination. BA treatment did not affect the embryo germination solely. But, the effect of BA and different concentrations of IAA was significant on embryo germination. The best concentration of IAA with BA treatment was 1 mg L<sup>-1</sup> and without BA was 3 mg L<sup>-1</sup>. The highest embryo germination rate was observed in 'Askari×Ruby Seedless' cross that was pretreated with BA and cultured on medium with 1 mg L<sup>-1</sup> IAA concentration.
Benzyladenine,Culture medium,Embryo rescue,Grapevine,Seedlessness
1023
1031
http://jast.modares.ac.ir/browse.php?a_code=A-23-11598-98&slc_lang=en&sid=23
M.
Razi
M.
Razi
100319475328460046722
100319475328460046722
No
Department of Horticulture, Urmia University, P. O. Box: 165, Urmia, Islamic Republic of Iran.
Department of Horticulture, Urmia University, P. O. Box: 165, Urmia, Islamic Republic of Iran.
R.
Jalili Marandi
R.
Jalili Marandi
100319475328460046721
100319475328460046721
Yes
Department of Horticulture, Urmia University, P. O. Box: 165, Urmia, Islamic Republic of Iran.
Department of Horticulture, Urmia University, P. O. Box: 165, Urmia, Islamic Republic of Iran.
H.
Doulati Baneh
H.
Doulati Baneh
100319475328460046724
100319475328460046724
No
Department of Seed and Plant Improvement, West Azerbaijan Agricultural and Natural Resources Research Center, P. O. Box: 365, Urmia, Islamic Republic of Iran.
Department of Seed and Plant Improvement, West Azerbaijan Agricultural and Natural Resources Research Center, P. O. Box: 365, Urmia, Islamic Republic of Iran.
B.
Hosseini
B.
Hosseini
100319475328460046663
100319475328460046663
No
Department of Horticulture, Urmia University, P. O. Box: 165, Urmia, Islamic Republic of Iran.
Department of Horticulture, Urmia University, P. O. Box: 165, Urmia, Islamic Republic of Iran.
R.
Darvishzadeh
R.
Darvishzadeh
100319475328460046457
100319475328460046457
No
Institute of Biotechnology, Urmia University, Urmia, Islamic Republic of Iran.
Institute of Biotechnology, Urmia University, Urmia, Islamic Republic of Iran.