Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1393
6
1
gregorian
2014
9
1
16
5
online
1
fulltext
en
Plant Regeneration from Protoplasts of Lilium ledebourii (Baker) Boiss
For half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. Somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. For this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from protoplasts. Protoplast isolation method was previously developed in <em>Lilium ledebourii</em> (Baker) Boiss. In this study, several valuable experiments were done based on completely randomized design with 3 replications and also each experiment was repeated twice. The results revealed that cell wall and colony formation were better in a liquid medium than those on a semi-solid medium. The highest plating efficiency (1.34×10<sup>6</sup> per gr FW) and callus formation was obtained by using a medium containing 1 mg L<sup>-1</sup> 2,4-D, 0.2 mg L<sup>-1</sup> Kin and 2 g L<sup>-1</sup> Yeast extract. Micro calli were formed after one month of culture. Many plantlets were formed on the calli after transfer of the proliferated calli to regeneration medium. The highest plantlet regeneration (91.66%) was obtained by using a medium containing 0.5mg L<sup>-1</sup> NAA, 1.5 mg L<sup>-1</sup> BA. Means comparison revealed that the semi- solid MS medium containing 0.5 mg L<sup>-1</sup> NAA and 1.5 mg L<sup>-1</sup> BA had the highest percentage of regeneration (91.66%), bulb number (8.83), and length (0.7366 cm), root <span class="hps">length</span> (0.421cm) <span class="hps">and </span>leaf number (13.66) and length (0.5052cm).
Callus formation,In-vitro culture,medium,Proliferated calli
1133
1144
http://jast.modares.ac.ir/browse.php?a_code=A-23-1000-5581&slc_lang=en&sid=23
S. K.
Tahami
S. K.
Tahami
100319475328460052698
100319475328460052698
No
Department of Horticultural Science, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Islamic Republic of Iran.
Department of Horticultural Science, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Islamic Republic of Iran.
E.
Chamani
E.
Chamani
100319475328460052704
100319475328460052704
Yes
Department of Horticultural Science, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Islamic Republic of Iran.
Department of Horticultural Science, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Islamic Republic of Iran.
N.
Zare
N.
Zare
100319475328460052152
100319475328460052152
No
Department of Horticultural Science, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Islamic Republic of Iran.
Department of Horticultural Science, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Islamic Republic of Iran.