Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1394
4
1
gregorian
2015
7
1
17
4
online
1
fulltext
en
Effects of Exogenous GA3 on Wheat Cold Tolerance
To clarify the underlying physiological mechanism of gibberellic acid (GA) in cold tolerance, the effects of exogenous GA<sub>3</sub> on malondialdehyde (MDA), osmoregulation substances and endogenous hormones levels in the tiller nodes of two wheat cultivars, namely, Dongnongdongmai 1 (a cold insensitive cultivar) and Jimai 22 (a cold sensitive cultivar), were investigated at three periods of cold winter (0, -10, -25°C). The results showed that low concentrations of GA<sub>3</sub> (0.1 and 1 µM) decreased the endogenous GA concentration in both cultivars, but only increased the abscisic acid (ABA)/GA ratio in Dongnongdongmai 1. High concentrations of GA<sub>3</sub> (10 and 100 µM) increased the MDA level, retarded the accumulation of soluble protein and sugar in both cultivars, but decreased the content of ABA and the ratio of ABA/GA only in Dongnongdongmai 1 and had no influence on those in Jimai 22. The re-greening rate of Dongnongdongmai 1 decreased as the concentration of exogenous GA<sub>3 </sub>increased. Correlation analyses showed that MDA was negatively correlated with re-greening rate, while soluble protein, sugar ABA content, and ABA/GA ratio were positively correlated with re-greening rate. In conclusion, low exogenous GA<sub>3</sub> level could decrease endogenous GA content and elevate ABA/GA ratio and soluble protein content, which help to improve cold tolerance. However, high exogenous GA<sub>3</sub> level decreased the ABA content and ABA/GA ratio, resulting in lower soluble sugar and protein content and aggravated oxidative damage, and finally weakened cold tolerance. The endogenous GA metabolism and ABA/GA balance play central roles in exogenous GA<sub>3 </sub>mediated cold tolerance.
Cold stress,Physiological mechanism,Phytohormone,Tiller node,Winter wheat
921
934
http://jast.modares.ac.ir/browse.php?a_code=A-23-1000-5104&slc_lang=en&sid=23
X.
Wang
X.
Wang
100319475328460057480
100319475328460057480
No
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
Ch.
Xu
Ch.
Xu
100319475328460057457
100319475328460057457
No
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
J.
Cang
J.
Cang
100319475328460057456
100319475328460057456
Yes
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
Y.
Zeng
Y.
Zeng
100319475328460057479
100319475328460057479
No
Communication and Electronic Engineering Institute, Qiqihar 161006, People’s Republic of China.
Communication and Electronic Engineering Institute, Qiqihar 161006, People’s Republic of China.
J.
Yu
J.
Yu
100319475328460057478
100319475328460057478
No
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
L.
Liu
L.
Liu
100319475328460057477
100319475328460057477
No
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
D.
Zhang
D.
Zhang
100319475328460057459
100319475328460057459
No
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
J.
Wang
J.
Wang
100319475328460057458
100319475328460057458
No
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.
College of Life Sciences, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China.