Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1396
2
1
gregorian
2017
5
1
19
3
online
1
fulltext
en
Exploring Possible Variation among Iranian <i>Erwinia amylovora</i> Strains Using Multilocus Typing and Tandem Repeat Analysis
In the current study, molecular typing of 50 <em>Erwinia amylovora</em> strains related to different regions in Iran was evaluated using multi-locus sequence analysis and variable number of tandem repeats. In the first assay, phylogenetic tree based on partial sequences of recombinase A, sigma factor S and a heat shock protein GroEL showed significant identity in studied gene sequences. A single nucleotide variation in <em>gro</em>EL was determined in IrGh59 strain related to <em>Crataegus</em> spp. from Ghazvin Province. In VNTR analysis, the same fingerprinting profile similar to <em>E. amylovora</em> reference strain ATCC49946 was yielded for tested strains except NBQ1 and MQ1 which may reflect a unique contaminating source for this disease in Iran. In addition, the honey-bee movements with respect to blossom season probably have a considerable role in fire blight unique dispersal in our area. The NBQ1 and MQ1 strains generated different VNTR profiles, isolated from cultivars NeishabourandEsfehan of<em> Cydonia oblonga</em> plant, respectively. No definite assessment can be expressed in this case. However, possible entry of other infection mass from neighboring countries should be determined. Overall, VNTR profile analyses are recommended as a tool to evaluate genetic differences in <em>E. amylovora</em> populations. In addition, employing more strains from different known sources <strong>could be assistance</strong> to achieve more accurate results about <em>E. amylovora</em> genetic variation and also fire blight distribution patterns.
Fire blight,Housekeeping genes,Iran,VNTR
745
754
http://jast.modares.ac.ir/browse.php?a_code=A-23-10091-1&slc_lang=en&sid=23
M.
Refahi
M.
Refahi
100319475328460060462
100319475328460060462
No
Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, P. O. Box: 91775-1163, Mashhad, Islamic Republic of Iran.
Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, P. O. Box: 91775-1163, Mashhad, Islamic Republic of Iran.
S.
Baghaee Ravari
S.
Baghaee Ravari
100319475328460060461
100319475328460060461
Yes
Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, P. O. Box: 91775-1163, Mashhad, Islamic Republic of Iran.
Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, P. O. Box: 91775-1163, Mashhad, Islamic Republic of Iran.
E.
Mahdikhani-Moghaddam
E.
Mahdikhani-Moghaddam
100319475328460060460
100319475328460060460
No
Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, P. O. Box: 91775-1163, Mashhad, Islamic Republic of Iran.
Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, P. O. Box: 91775-1163, Mashhad, Islamic Republic of Iran.