Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1389
4
1
gregorian
2010
7
1
12
3
online
1
fulltext
en
Gut PH, and Isolation and Characterization of Digestive
α-D-Glucosidase of Sunn Pest
A study of insect digestive enzymes makes sense given that the gut is the major interface between the insect and its environment. An understanding of gut and digestive enzyme function is essential when advanced methods of insect management such as application of enzyme inhibitors and transgenic plants are developed to control insect pests. The aim of the current research project was to study midgut anatomy, midgut pH and α-glucosidase activity in Sunn pest, Eurygaster integriceps Puton (Hemip: Scutelleridae). Sunn pest midgut is comprised of four distinct regions including first ventriculus (V1), second ventriculus (V2), third ventriculus (V3) and finally fourth ventriculus (V4). The study showed that the first three regions of the Sunn pest midgut are more acidic (pH= 5–5.2), the fourth region moderately acidic (pH= 6.2–6.4), and hindgut slightly acidic (pH= 6.5–6.8). Enzyme assay showed that α-glucosidase activity is present in midgut and salivary glands of adult E. intergriceps. The specific activity of midgut enzyme was 0.17 U mg protein-1 while the specific activity of the salivary glands enzyme was 0.033 U mg protein-1. Optimum temperature and pH values for α-glucosidase were determined to be 40-45°C and 5, respectively. Based on linear regression analysis of reciprocal p-nitrophenyl substrate (p-nitrophenyl α-D-glucopyranoside) concentration versus reciprocal α-glucosidase activity Km and Vmax were 17 and 0.9 mM p-nitrophenol min-1, respectively. The effect of different ion concentrations on α-glucosidase activity showed that Na+, K+, Mg2+, and Ca2+ ions exerted positive effects on the enzyme activity. Other compounds tested such as urea, SDS, Tween 80, Triton X-100 and EDTA had an inhibitory effect on enzyme activity.
pH,α-glucosidase,Gut,Sunn pest
265
274
http://jast.modares.ac.ir/browse.php?a_code=A-10-1000-4294&slc_lang=en&sid=23
A. R.
Bandani
A. R.
Bandani
100319475328460044865
100319475328460044865
Yes
Department of Plant Protection, School of Plant Protection and Horticultural Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Islamic Republic of Iran.
Department of Plant Protection, School of Plant Protection and Horticultural Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Islamic Republic of Iran.
M.
Kazzazi
M.
Kazzazi
100319475328460044866
100319475328460044866
No
Department of Plant Protection, School of Plant Protection and Horticultural Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Islamic Republic of Iran.
Department of Plant Protection, School of Plant Protection and Horticultural Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Islamic Republic of Iran.
M.
Allahyari
M.
Allahyari
100319475328460044867
100319475328460044867
No
Department of Plant Protection, School of Plant Protection and Horticultural Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Islamic Republic of Iran.
Department of Plant Protection, School of Plant Protection and Horticultural Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Islamic Republic of Iran.