Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1395
12
1
gregorian
2017
3
1
19
2
online
1
fulltext
en
Pollen Ultrastructural Image Analysis among Ancient Native Olive Genotypes in the Central Eastern Tunisia
The olive tree (<em>Olea europaea</em> L.) is considered as one of the oldest and the most important fruit crops of the Mediterranean basin, which is characterized by the existence of a considerable number of different olive cultivars. Therefore, the olive cultivar identification is crucial to safeguard the genetic patrimony of this species. Different morphological and molecular markers were used to discriminate among cultivars. The aim of the present work was to describe different pollen morphological and ultrastructural parameters (shape, size and exine pattern) as an additional tool for the identification of olive cultivars. Observations were carried on seventy centennial olive accessions grown in the Central Eastern part of Tunisia using Scanning Electron Microscopy (SEM) and Image analysis (ImageJ). Pollen were three-zonocolpate and elliptical-prolate or subprolate. Pollen morphological qualitative traits revealed specific differences among the studied genotypes including variation in whole grain shape and also exine pattern ornamentation as meshes profile and regularity and muri thickness. The quantitatively measured traits were significantly different among pollen from diverse genotypes. Polar and equatorial diameters varied from 21.80 to 29.88 µm and from 14.47 to 21.14 µm, respectively, while the pollen area ranged between 274.58 and 466.35 µm<sup>2</sup>. Frequency distributions of most measured pollen parameters depicted a normal distribution. The three principal components of the Principal Component Analysis (PCA) accounted for more than 97% of the total variation. The first Principal Component (PC1) was correlated to pollen size. The second (PC2) and the third (PC3) were correlated to exine texture and to pollen shape, respectively. Both morphometric features and exine pattern observations were potentially relevant tools to discriminate among the studied genotypes. Further combination between pollen ultrastructural analysis, morphological and molecular markers is fully desirable, in subsequent work, to improve both reliability and discriminative ability for cultivars classification.
Exine pattern,Olea europaea L,Pollen micromorphology,Scanning electron. Microscopy
401
413
http://jast.modares.ac.ir/browse.php?a_code=A-23-15942-1&slc_lang=en&sid=23
I.
Laaribi
I.
Laaribi
100319475328460048294
100319475328460048294
Yes
High Agronomic Institute, IRESA-University of Sousse, 4042 Chott-Mariem, Tunisia.
High Agronomic Institute, IRESA-University of Sousse, 4042 Chott-Mariem, Tunisia.
M.
Mezghani Aiachi
M.
Mezghani Aiachi
100319475328460048293
100319475328460048293
No
Olive Tree Institute, B.P. 40, 4061 Sousse, Tunisia.
Olive Tree Institute, B.P. 40, 4061 Sousse, Tunisia.
H.
Gouta
H.
Gouta
100319475328460048292
100319475328460048292
No
Olive Tree Institute, B.P. 40, 4061 Sousse, Tunisia.
Olive Tree Institute, B.P. 40, 4061 Sousse, Tunisia.
C.
Giordano
C.
Giordano
100319475328460048291
100319475328460048291
No
Center of Electron Microscopy “Laura Bonzi”, ICCOM, National Research Council (CNR), Firenze, Italy.
Center of Electron Microscopy “Laura Bonzi”, ICCOM, National Research Council (CNR), Firenze, Italy.
M. C.
Salvatici
M. C.
Salvatici
100319475328460048290
100319475328460048290
No
Center of Electron Microscopy “Laura Bonzi”, ICCOM, National Research Council (CNR), Firenze, Italy.
Center of Electron Microscopy “Laura Bonzi”, ICCOM, National Research Council (CNR), Firenze, Italy.
F.
Labidi
F.
Labidi
100319475328460048289
100319475328460048289
No
Olive Tree Institute, B.P. 40, 4061 Sousse, Tunisia.
Olive Tree Institute, B.P. 40, 4061 Sousse, Tunisia.
M.
Mars
M.
Mars
100319475328460048288
100319475328460048288
No
High Agronomic Institute, IRESA-University of Sousse, 4042 Chott-Mariem, Tunisia.|Regional Research Center for Horticulture and Organic Agriculture (CRRHAB), B. P. 57, 4042 Chott Mariem, Sousse, Tunisia.
High Agronomic Institute, IRESA-University of Sousse, 4042 Chott-Mariem, Tunisia.|Regional Research Center for Horticulture and Organic Agriculture (CRRHAB), B. P. 57, 4042 Chott Mariem, Sousse, Tunisia.