Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1394
2
1
gregorian
2015
5
1
17
3
online
1
fulltext
en
Antimicrobial and Anti-oxidative Effects of Methanolic Extract of Dorema aucheri Boiss.
<em><span dir="RTL"> </span>Dorema aucheri</em> Boiss. (Apiaceae) is an endemic plant growing in Iran. This plant is used as food and its extracts are recommended for the treatment of a wide range of diseases. To conduct this study, leaf, stem, and flower of <em>D. aucheri</em> were collected from near Yasouje, Iran. Antioxidant and antimicrobialeffects of methanol extracts were measured. Further, total phenolic,flavonoid, anthocyanin, carotenoid, soluble sugar, <span class="hps"><span lang="EN">gallic</span></span><span class="hps"><span lang="EN">acid</span>,</span><span class="longtext"><span lang="EN">chlorogenic acid,</span></span><span class="hps"><span lang="EN">caffeic</span></span><span class="hps"><span lang="EN">acid, and</span></span><span class="hps"><em><span lang="EN">p</span></em></span><span class="hps"><span lang="EN">-</span></span><span class="longtext"><span lang="EN">coumaric acid </span></span>contentsof plant methanol extracts were also determined. The results showed that total phenolic, anthocyanin, and soluble sugar in the stem of <em>D. </em><em>aucheri</em> were 22.72 mg GAE g<sup>-1</sup> dW, 19.33 mg g<sup>-1</sup> dW, and 6.45 mg g<sup>-1</sup> dW respectively, greater than those of the other samples tested. Also, phenolic acids were identified by RP-HPLCand chlorogenic acid was the predominant phenolic compound in the samples. The highest amount of flavonoid (1.95 mg QE g<sup>-1</sup> dW) was observed in the flower. All of the different extracts exhibited a good antioxidant activity based on inhibition of fatty acid oxidation assay. Themaximal inhibition was observed in the leaf (48.52%) and flower (54.24%) of <em>D. aucheri</em>. Ferric reducing antioxidant power (FRAP) was also high in the stem and flower. In addition, the results showed that leaf, stem, and flower extracts had inhibitory activity against four bacteria tested. The highest antimicrobial activity was obtained with flower extract. These results suggest that the methanolic extracts from different parts of <em>D. aucheri</em> are a valuable source of effective compounds. The antioxidant and antimicrobial activity of extracts was correlated with <em>p</em>-coumaric and caffeic acid content.
Antioxidant,Antimicrobial activity,Dorema aucheri,phenolic compounds
623
634
http://jast.modares.ac.ir/browse.php?a_code=A-23-1000-2338&slc_lang=en&sid=23
M.
Mianabadi
M.
Mianabadi
100319475328460056803
100319475328460056803
Yes
Department of Biology, Faculty of Science, Golestan University, Gorgan, Islamic Republic of Iran.
Department of Biology, Faculty of Science, Golestan University, Gorgan, Islamic Republic of Iran.
M.
Hoshani
M.
Hoshani
100319475328460056804
100319475328460056804
No
Department of Biology, Faculty of Science, Golestan University, Gorgan, Islamic Republic of Iran.
Department of Biology, Faculty of Science, Golestan University, Gorgan, Islamic Republic of Iran.
S.
Salmanian
S.
Salmanian
100319475328460054802
100319475328460054802
No
Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources,
Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources,