Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1391
2
1
gregorian
2012
5
1
14
3
online
1
fulltext
en
Allelic Polymorphism of Makoei Sheep Calpastatin Gene Identified by Polymerase Chain Reaction and Single Strand Conformation Polymorphism
Calpastatin (CAST) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. In the present study, the polymorphism of the CAST gene of Makoei sheep was investigated by polymerase chain reaction and single strand conformation polymorphism technique (PCR–SSCP). Genomic DNA was extracted from whole blood samples collected from 100 sheep. A 622 bp CAST exon 1 segment was amplified by standard PCR, using the locus specific primers. PCR products were subjected to a non-denaturing gel electrophoresis. Four SSCP patterns, representing four different genotypes, were identified. The frequencies of the observed genotypes were 0.31, 0.04, 0.63 and 0.02 for AA, BB AB and AC, respectively. Allele frequencies were 0.6313, 0.3586 and 0.01 for A, B and C, respectively. The Observed heterozygosity (Hobs) value for CAST gene was 0.4728. The chi-square test showed significant (P< 0.01) deviation from Hardy-Weinberg equilibrium for this locus in Makoei sheep population.
CAST gene,Makoei sheep,PCR,SSCP
533
538
http://jast.modares.ac.ir/browse.php?a_code=A-23-1000-1902&slc_lang=en&sid=23
M.
Ranjbari
M.
Ranjbari
100319475328460046062
100319475328460046062
No
Institute for Biotechnology, Department of Cellular and Molecular Biology, Urmia University, Urmia, Islamic Republic of Iran.
Institute for Biotechnology, Department of Cellular and Molecular Biology, Urmia University, Urmia, Islamic Republic of Iran.
A.
Hashemi
A.
Hashemi
100319475328460046061
100319475328460046061
No
Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, West Azerbaijan, Islamic Republic of Iran.
Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, West Azerbaijan, Islamic Republic of Iran.
K.
Mardani
K.
Mardani
100319475328460046059
100319475328460046059
Yes
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Islamic Republic of Iran.
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Islamic Republic of Iran.
R.
Darvishzadeh
R.
Darvishzadeh
100319475328460046063
100319475328460046063
No
Department of Plant Breeding, Faculty of Agriculture, Urmia University, Urmia, Islamic Republic of Iran.
Department of Plant Breeding, Faculty of Agriculture, Urmia University, Urmia, Islamic Republic of Iran.