Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1391
10
1
gregorian
2013
1
1
15
1
online
1
fulltext
en
In Vitro and In Vivo Induction, and Characterization of Beauvericin Isolated from Beauveria bassiana and Its Bioassay on Galleria mellonella Larvae
Entomopathogenic fungi produce secondary metabolites which may bioactively help fungus in its virulence toward insect hosts.<em> Beauveria</em> <em>bassiana</em> produces several toxic low molecular compounds <em>in vitro</em> as well as <em>in vivo</em>, the most important of them is Beauvericin. The BEH isolate of the fungus was selected for Beauvericin assay. Beauvericin was obtained from surface and submerged cultures of the fungus in PDB and PDA, culture filtrates, and <em>in vivo</em> conidia harvested from insect cadavers. Results indicated that <em>in vivo</em> fungal conidia contained the most Beauvericin, causing a higher mortality to <em>Galleria mellonella</em> larvae as compared with <em>in vitro</em> fungal products in their different concentrations. Beauvericin chromatogram revealed that Beauvericin was in its greatest quantity in comparison with the other secondary metabolites of BEH isolate. The impact of Beauvericin on mean larval survival and on paralysis time was in agreement with bioassay data showing lower ST<sub>50</sub> <em>vs.</em> higher PT<sub>50</sub> in larvae treated with metabolites of the insect-derived conidia.
Beauveria bassiana,bioassay,Galleria mellonella,Mass Spectrometry
1
10
http://jast.modares.ac.ir/browse.php?a_code=A-23-11598-145&slc_lang=en&sid=23
S. A.
Safavi
S. A.
Safavi
100319475328460046391
100319475328460046391
Yes
Department of Plant Protection, College of Agriculture, Urmia University, Urmia, Islamic Republic of Iran.
Department of Plant Protection, College of Agriculture, Urmia University, Urmia, Islamic Republic of Iran.