Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1392
10
1
gregorian
2014
1
1
16
1
online
1
fulltext
en
Genetic Characterization, Polymorphism and Evolution Analysis in Part of the EDNRB Gene in Cattle and Goat
The aim of this study was to analyze the polymorphisms existing in the 5´ flanking region, exonic, and some parts of the intronic regions as well as methylation analysis of the <em>EDNRB</em> gene in cattle and goat. These regions were sequenced in three different breeds of cattle including Sistani, Golpayegani, and Holstein and were compared with that of Marghoz goat. The results identified that this gene encodes 441 amino acid residues in cattle and goat with highly conserved domains between them. A total of 55 transition and 43 transversion mutations were observed in the 5´ and 3´ flanking and exonic regions. Among the six coding mutations in goat <em>EDNRB</em> gene, a missense mutation of C236>T resulted in substitution of Alanine-79 to Valine. The frequency of the T allele in the goat population was 0.77. In addition, an insertion of TG<sub>(29)</sub> repeat was identified at the -773 upstream of the gene in goat samples. The promoter region of the gene harbors two GC rich conserved motifs and transcription factor binding sites for GATA and SP1 in both species, which may modulate the expression of the gene. The methylation analysis of a 182 bp fragment of the 5´ region of the <em>EDNRB</em> gene, located at the fringe of the CpG island, was found to be completely methylated in both cattle and goat. Evolutionary analysis revealed that the cattle and goat EDNRB proteins were sisters in their own clade and d<sub>N / </sub>d<sub>S</sub> ratio demonstrated that the <em>EDNRB</em> gene underwent purifying selection during evolution.
Methylation,Mutation,Promoter,Selection
79
90
http://jast.modares.ac.ir/browse.php?a_code=A-23-11598-140&slc_lang=en&sid=23
A. A.
Masoudi
A. A.
Masoudi
100319475328460050222
100319475328460050222
Yes
Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Islamic Republic of Iran.
Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Islamic Republic of Iran.