Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1386
1
1
gregorian
2007
4
1
9
2
online
1
fulltext
en
Ultrastructure and Osmoregulatory Function of The
Branchial Chamber in the Larvae of Dragonfly,
Libellula lydia (Odonata)
The ultrastructure of the cells, Na+, K+-ATPase activity and immunolocalization were examined in the barnchial chamber of Libellula lydia (Drury, 1773) larvae. Na+,K+-ATPase activity and localization were performed through biochemical techniques and immunofluorescence light microscopy using a mouse monoclonal antibody IgGα5, respectively. The branchial chamber possesses six pair gills lamellae that extend into the rectal lumen. A thickened epithelial layer and a modified fat body cells layer are present at the base of the each gill lamella. Epithelial cells covered by a thin cuticle and they possess apical microvilli and baso-lateral membrane infoldings associated with mitochondria. The cytoplasm of the modified fat body cells is filled with mitochondria, glycogen and a few lipid droplets. The Na+,K+-ATPase activity was significantly higher (15.36 µM Pi mg-1 protein h-1) in the branchial chamber. Na+,K+-ATPase immunofluorescence staining was observed in the epithelial layer cells of the basal pads of the rectal gill lamellae, with a consistently high immunoreactivity. These findings show that the epithelial cells present cytological features of the ionocytes, a high activity and concentration of Na+,K+-ATPase, confirming their participation in osmoregulation through active ion exchanges.
Anisoptera,Branchial chamber,Dragonfly,Immunolocalization,Larvae,Na+,K+-ATPase
119
127
http://jast.modares.ac.ir/browse.php?a_code=A-10-1000-8893&slc_lang=en&sid=23
S.
Khodabandeh
S.
Khodabandeh
100319475328460045352
100319475328460045352
Yes
Faculty of Marine Sciences, University of Tarbiat Modarres, Tehran, Islamic Republic of Iran.
Faculty of Marine Sciences, University of Tarbiat Modarres, Tehran, Islamic Republic of Iran.