Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1387
1
1
gregorian
2008
4
1
10
2
online
1
fulltext
en
The Effects of Entomopathogenic Fungus, Tolypocladium cylindrosporum on Cellular Defence System of Galleria mellonella
The entomopathogenic-fungus-life cycle is associated with the synthesis and secretion of a number of toxic metabolites, including extracellular enzymes and the low-molecular weight compound (toxin). The potential for a successful pathogen relies on the ability to overcome the various host-defence systems. Interaction between the fungus, Tolypocla-dium (Deuteromycetes), its secondary metabolite, and its host cellular defence were inves-tigated using in vivo and in vitro studies. In vitro studies showed that toxins (efrapeptins) inhibit phagocytic activity of Galleria mellonella (Lep: Pyralidae) haemocytes. The effect of efrapeptins on phagocytosis was in a dose-dependent manner i.e. the amount of phago-cytosis in a treated cell-culture with 0, 3, and 30 g efrapeptins per well was about 12, 7.5, and 4.5 %, respectively (P<0.05). In vivo studies showed that injection of insects with 0, 0.25, and 0.025 g toxin rendered percentages of phagocytosis of 13, 11.5, and 7.2, respec-tively (P<0.05). There was no significant reduction in the total haemocyte count (THC) when larvae were injected with Tolypocladium cylindrosporum spores until 24 hours fol-lowing injection. However, THC was suppressed at 48 hours post-treatment of larvae with spores. Considering that toxin suppresses phagocytosis, nodule formation, but not THC, this study suggests that efrapeptins may interfere with the ligand-receptor interactions that are likely to occur in the plasma membrane of specific haemocytes.
Entomopathogenic fungi,Cellular defence system,Efrapeptins,Galleria mellonella,Interactions
135
146
http://jast.modares.ac.ir/browse.php?a_code=A-10-1000-7893&slc_lang=en&sid=23
A. R.
Bandani
A. R.
Bandani
100319475328460045245
100319475328460045245
Yes
Department of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj, Islamic Republic of
Iran.
Department of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj, Islamic Republic of
Iran.