Journal of Agricultural Science and Technology
Journal of Agricultural Science and Technology
JAST
Agriculture
http://jast.modares.ac.ir
1
admin
1680-7073
2345-3737
10.22034/jast
en
jalali
1379
1
1
gregorian
2000
4
1
2
2
online
1
fulltext
en
Serological Detection and Symptomology of TomatoSpotted Wilt Virus in Tehran Province, Iran
ABSTRACT Tomato spotted wilt virus (TSWV) was detected in tomato leaf and fruit samples collected from Viiramin reyion in Tehran province using indicator test plants including Petunia hybrida, Nicatiana glutinosa, N. tabacum cv. Snmsun NN, N. clevelandit and N. benthamiana and seroloyical tests. Small browinsh local necrotic lesions appeared on P. hybrida leaves 2-4 days post-inoculation. Systemic symptoms included concentric ring spots on leaves, stem necrosis, wilting and tissue collapse of plants 7-10 days following the inoculation. Among 145 tomato samples collected from Ghazvin, Hashtgerd, Karaj, Malard, Shahriar and Varamin in Tehran province, only Varamin samples were infected with TSWV using EL1SA, DIBA and SSEM. TSWV host range specificity and symptom expression were tested on Capsicum annuum L., Chenopodium amaranticolor I«, Citrullus vulgaris L., Oicumis melo var. inodorus, C. mela var. reticukUus, C. salivas L., Lycopersicon esculentum Mill., Phasenlus vulgaris L., Solatium melongena 1- and S. tuberosum. Typical symptoms on these plants included concentric ring spots, chlorosis, vein clearing, tissue necrosis, stunting and local lesion formation. Antiserum prepared against a partially purified TSWV preparation cross-reacted with TSWV-infected tomato samples.
Tomato,Tomato Spotted Wilt Virus,SSEM,DAS-ELISA,DAS-DIBA
107
117
http://jast.modares.ac.ir/browse.php?a_code=A-10-1000-7732&slc_lang=en&sid=23
M.
Mohammadi
M.
Mohammadi
100319475328460044895
100319475328460044895
Yes
Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
A.
Esmaeeli-far
A.
Esmaeeli-far
100319475328460044896
100319475328460044896
No
Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
J.
Zad
J.
Zad
100319475328460044897
100319475328460044897
No
Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
Gh.
Mossahebi
Gh.
Mossahebi
100319475328460044778
100319475328460044778
No
Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
M.
Okhovat
M.
Okhovat
100319475328460044898
100319475328460044898
No
Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.