RT - Journal Article T1 - Genetic Diversity of Stone Fruit Cultivars Preserved On-Farm in Southern Spain JF - mdrsjrns YR - 2019 JO - mdrsjrns VO - 21 IS - 4 UR - http://jast.modares.ac.ir/article-23-13725-en.html SP - 943 EP - 955 K1 - Genetic resources K1 - Homonyms Local varieties K1 - Microsatellites K1 - Prunus. AB - Old traditional cultivars are valuable genetic resources for crop improvement, but a great number of them have disappeared in the past century. This study aimed to characterize traditional cultivars of different Prunus species collected in small family orchards in southwestern Spain and to evaluate their genetic diversity and relationships. One hundred and twelve accessions belonging to 36 traditional cultivar denominations were analyzed using eight SSR loci transferable across the genus Prunus. The most useful loci to analyze different Prunus species were UDP96-005, BPPCT-002, UDP98-410 and ps02a12. A total of 152 alleles were observed, and 112 were unique to certain species. Sixty-eight different genotypes were found, revealing the possible existence of homonyms among traditional cultivar names. The clustering analysis was consistent with the taxonomic classification of the different species studied and with the geographical origins of the accessions within each species. The results showed wide genetic variability of traditional cultivars of stone fruits grown in small family orchards, which highlights the need to preserve them using both in-situ and ex-situ strategies. Twenty-eight of these accessions are currently conserved ex-situ at the University of Sevilla, Spain. The use of highly transferable SSRs has been proven as efficient in multi-species surveys performed on-farm. LA eng UL http://jast.modares.ac.ir/article-23-13725-en.html M3 ER -