TY - JOUR T1 - Polyphasic Approach Used for Distinguishing Fusarium temperatum from Fusarium subglutinans TT - استفاده ازرویکرد چند فازی برای تشخیصFusarium temperatum از Fusarium subglutinans JF - mdrsjrns JO - mdrsjrns VL - 21 IS - 1 UR - http://jast.modares.ac.ir/article-23-12766-en.html Y1 - 2019 SP - 221 EP - 232 KW - Maize KW - Maximum parsimony analysis KW - Polyphasic approach of identification KW - Sorghum seed. N2 - Morphological, biological, and phylogenetic approaches were undertaken for the identification of pathogenic species F. temperatum in the Serbian population of F. subglutinans collected in the 1999-2010 period from Zea mays (3 root, 15 stalk, and 6 seed samples), Sorghum bicolor (two seed samples), Hordeum vulgare (one seed sample) and Taraxacum officinale (one seed sample). Based on interspecies mating compatibility analyses and the maximum parsimony analysis of EF-1α sequences, only two strains, originating from S. bicolor seed (MRIZP 0418 and MRIZP 0552), were identified as F. temperatum, while the remaining 26 single-spore strains were identified as F. subglutinans Group 2. In situ detached barley leaf assay and artificially stalk and ear inoculation of two maize hybrids demonstrated that both F. temperatum and F. subglutinans strains were medium and strong pathogens under laboratory and field conditions, respectively. These are the first data on the F. temperatum as seed-borne pathogens of sorghum, as well as pathogenicity of F. temperatum strains on maize. M3 ER -