%0 Journal Article %A Ghanizadeh, H. %A Harrington, K. %A James, T. %A Woolley, D. %A Ellison, N. %T Restricted Herbicide Translocation Was Found in Two Glyphosate-resistant Italian Ryegrass (Lolium multiflorum Lam.) Populations from New Zealand %J Journal of Agricultural Science and Technology %V 18 %N 4 %U http://jast.modares.ac.ir/article-23-3456-en.html %R %D 2016 %K EPSP synthase mutation, Glyphosate, Herbicide resistance, Mechanism of resistance, Weeds, %X Glyphosate resistance has been found in two populations of Italian ryegrass (Lolium multiflorum) following many years of glyphosate application in New Zealand vineyards. Dose-response experiments showed that both glyphosate-resistant Italian ryegrass populations had 10-fold levels of resistance to glyphosate compared to a susceptible population. Possible mechanisms of glyphosate resistance target site mutation at position Pro-106 of 5-enolpyruvyl-shikimate-3-phosphate synthase gene and alterations in glyphosate absorption/translocation in these populations were investigated. Genotyping assays demonstrated that there was no point mutation at Codon 106 of the 5-enolpyruvyl-shikimate-3-phosphate synthase gene in either of the resistant populations. Glyphosate-resistant and susceptible populations did not differ in 14C-glyphosate absorption. However, in both resistant populations, much more of the absorbed 14C-glyphosate was retained in the treated leaf than occurred in the susceptible population. Significantly more 14C-glyphosate was found in the pseudostem region of susceptible plants than resistant plants. Based on these results, it was suggested that alterations in glyphosate translocation patterns plays a major role in glyphosate resistance for Italian ryegrass populations from these New Zealand vineyards. %> http://jast.modares.ac.ir/article-23-3456-en.pdf %P 1041-1051 %& 1041 %! Restricted herbicide translocation was found in two glyphosate-resistant Italian ryegrass (Lolium multiflorum) populations from New Zealand %9 %L A-23-1000-2623 %+ Institute of Agriculture and Environment, Massey University, PB 11-222, Palmerston North 4442, New Zealand. %G eng %@ 1680-7073 %[ 2016