TY - JOUR T1 - Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS TT - روش سطح پاسخ جهت بهینه سازی پارامترهای هیدرولیز برای تولید پپتیدهای آنتی-اکسیدانت از پروتئین جوانه گندم با استفاده از آلکالاز. شناسایی پپتیدهای آنتی‌اکسیدانت با روش LC-MS/MS JF - mdrsjrns JO - mdrsjrns VL - 21 IS - 4 UR - http://jast.modares.ac.ir/article-23-17648-en.html Y1 - 2019 SP - 829 EP - 844 KW - ABTS assay KW - Amino acids profiles KW - Bioactive peptides KW - RSM. N2 - The antioxidant capacity of wheat germ protein hydrolyzed by Alcalase was optimized using Response Surface Methodology (RSM). The optimum hydrolyzing parameters were found at temperature of 52.28°C, time 233 minutes, and E/S 1.46 %. The amino acids profiles of intact and hydrolyzed proteins showed that Wheat Germ Protein Hydrolysate (WGPH) had higher percentage of hydrophobic amino acids than that of intact protein. WGPH prepared in optimum condition was fractionated by RP-HPLC. The obtained fractions were subjected to ABTS assay for antioxidant capacity evaluation. The fraction with higher antioxidant value was then exposed to further analysis by LC-ESI/MS/MS. The sequences of the peptides were found to be TVGGAPAGRIVME (1257.66 Da) and GNPIPREPGQVPAY (1494.77 Da). M3 ER -