Volume 3, Issue 3 (2001)                   JAST 2001, 3(3): 171-179 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ahmadi A, Baker D A. In vitro Sucrose Uptake and Starch Synthesis by Isolated Wheat (Triticum aestivuim) Grains. JAST 2001; 3 (3) :171-179
URL: http://jast.modares.ac.ir/article-23-259-en.html
1- Department of Agronomy and Plant Breeding, College of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
2- Department of Biological Sciences, Wye College, University of London, Wye, Ashford, Kent TN 25 5AH, UK.
Abstract:   (5442 Views)
The aim of this study was to establish and optimise a simplified method of halved grain culture, in order to utilise it to evaluate the ability of isolated sinks (developing wheat grains) in sucrose uptake and conversion (defined by sink strength) under unlimited source conditions. Both sucrose uptake and starch synthesis increased linearly over the incubation periods used in this study. A biphasic pattern of sucrose uptake in response to external sucrose concentrations was evident. Starch synthesis also increased curvilinearly with respect to external sucrose concentration. 2,4-dinitrophenol (DNP)) inhibited both sucrose uptake and starch synthesis. An increase in pH from 6.5 to 8 reduced both sucrose uptake and, to a lesser extent, starch synthesis. From these observations, a metabolically active uptake of sucrose by endosperm cells concomitant with a diffusional influx of sucrose into the endosperm cells were both evident. The relative importance of both the active and diffusional movement of sucrose and the importance of transport across the plasma membrane is discussed in the text.
Full-Text [PDF 194 kb]   (4505 Downloads)    
Subject: Agronomy
Received: 2010/05/16 | Accepted: 2010/05/16 | Published: 2010/05/16

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.