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The bioactive compounds of barberry such as water-soluble vitamins are used in medical and food industries. In this study, the effect of different extraction conditions and various process conditions were studied on water-soluble vitamins profile. The extraction conditions included varieties (species) are of fruits (B. integerrima: A, B. vulgaris: P), solvents (Water: W, Ethanol: E), light (Presence: L, Absence: T), pH (3 and 1.5), and temperature (25 and 50ºC). The process conditions included heating (95 and 80°C), chilling (ref 1, 2 months), freezing (con), microwave (mic) and gamma irradiation (γ at doses of 0.5, 1, 2.5, 5, 7.5, 10 kGy). The highest and lowest amounts of water-soluble vitamins in various extracts of different extraction conditions were respectively as follows: vitamin C in AWL1.550 (highest) and PEL350 (lowest); B₅ in PWL1.550 and AEL1.525, AWL1.550; B₆ in AWT350, and AEL1.550; B₁ in PET350 and PET1.550; folic acid in AET350 and PWT350; biotin in AEL350 and AEL1.550; B₂ in AET350 and PWL350 extracts. The highest and lowest amounts of water-soluble vitamins in various process conditions were respectively as follows: vitamin C (A_con 460 (highest), A_ref1 146.87 (lowest), P_con 242.96 (highest), P_mic 21.52 (lowest)), B₅ (A_γ0.5 2919.18, A₉₅ 1312.42, P_γ10 3110.88, P_ref2 1051.52), B₆ (A_con 36.30, A_γ7.5 21.04, P_γ10 12.70, P₉₅ 8.73), B₁ (A_con 2113.00, A_ref2 965.09, P_con 2298.15, P_γ10 217.76), folic acid (A_γ0.5 1700.38, A_γ10 947.11, P_con 104.78, P_γ10 in B. vulgaris after processing was not found), biotin (A_mic 2267.58, A_γ10 1404.63, P_con 324.72, P_γ2.5 118.33), and B₂ (A_γ0.5 586.43, A_γ10 274.34, P_con 297.19, P_γ10 6.79).
 

Keywords: B group vitamins, Bioactive compounds, HPLC, Vitamin C.

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