Volume 12, Issue 2 (2010)
JAST 2010, 12(2): 233-239 |
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1- Mycotoxins Research Laboratory, Plant Pests and Diseases Research Institute, Tehran, Islamic Republic of Iran.
2- Islamic Azad University, Tehran, Islamic Republic of Iran.
2- Islamic Azad University, Tehran, Islamic Republic of Iran.
Abstract: (6527 Views)
Deoxynivalenol contamination was determined in corn produced in Golestan and Ardabil (Moqan) Provinces, Iran, in 2004-2005. Samples were collected from different stages of production, including before harvest, at harvest, post harvest as well as after drying. Ground sub samples were extracted with water; each extracted sample was cleaned up through an immunoaffinity column. Deoxynivalenol was estimated through reversed-phase High Performance Liquid Chromatography (HPLC). The linearity of standard curve for 50 -10,000 ng ml-1 of standard solutions was proved (R2= 0.9999). Detection limit was 10 ng g-1. Recovery of the method for 1,000 and 500 ng g-1 spiked samples was 73.5% and 93.5% (n= 5). Deoxynivalenol contamination was found in 76.7% of samples in the range of 54.4-518.4 ng g-1. The mean of contamination was 116.25 ng g-1. This is the first report of natural DON contamination of corn from Iran.
Received: 2010/01/24 | Accepted: 2010/01/24 | Published: 2010/01/24
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