Volume 12, Issue 1 (2010)                   JAST 2010, 12(1): 79-89 | Back to browse issues page

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Keneni A, Assefa F, Prabu P C. Isolation of Phosphate Solubilizing Bacteria from the Rhizosphere of Faba Bean of Ethiopia and Their Abilities on Solubilizing Insoluble Phosphates. JAST. 12 (1) :79-89
URL: http://journals.modares.ac.ir/article-23-10330-en.html
1- Department of Applied Biology, Ambo University College, Ambo, Ethiopia.
2- Department of Biology, Addis Ababa University, Addis Ababa, Ethiopia.
Abstract:   (4327 Views)
Native phosphate solubilizing bacteria (PSB) were isolated from four areas (Ankober, Keyt, Mehalmeda and Molale) of Ethiopia to study their effect on releases of soluble phosphorus from insoluble P sources. The highest bacterial number was found at Keyt (2.6103 g-1soil) and the least at Molale (15 g-1soil). Five efficient PSB were selected for further study based on their ability in forming a higher clear zone diameter than the other isolates. These isolates were identified based on phenotypic characters as Pseudomonas sp. Anb-105, Meh-008, Meh-101, Meh-303 and Meh-305. The phosphate solubilizing efficiency of these five isolates along with Jim-41 isolate from the National Soil Research Centre were studied using different P sources [Tricalcium Phosphate (TCP), Egyptian Rock Phosphate (ERP), Bikilal Rock Phosphate (BRP) and Old Bone meal (OB)] in an incubation study. The results revealed that all the PSB isolates significantly (P 0.01) solubilized a higher amount of TCP, ERP and OB over the uninoculated control. The highest amount of solubilization was achieved for TCP with Meh-305 (39 mg per 50 ml) followed by ERP with Meh-101 (31 mg per 50ml) at pH 3.82 and 3, respectively. Although Meh-008 and Jim-41 isolates solubilized significant amount of BRP during the 20 days of incubation, the soluble P obtained was very small as compared to other P source tested.
Full-Text [PDF 488 kb]   (7621 Downloads)    
Subject: Soil Science
Received: 2010/01/24 | Accepted: 2010/01/24 | Published: 2010/01/24

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