Volume 23, Issue 3 (2021)
JAST 2021, 23(3): 711-725 |
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Jami H, Bayat H, Shams-Bakhsh M. Detection of Important Viruses on Dianthus Caryophillus L. in Markazi Province, Iran, and Molecular Analysis of Carnation Vein Mottle Virus. JAST 2021; 23 (3) :711-725
URL: http://jast.modares.ac.ir/article-23-36270-en.html
URL: http://jast.modares.ac.ir/article-23-36270-en.html
1- Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
2- Ornamental Plant Research Center, Institute of Horticultural Sciences, Agriculture Research, Education and Extension Organization (AREEO), Islamic Republic of Iran.
3- Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran. ,shamsbakhsh@modares.ac.ir
2- Ornamental Plant Research Center, Institute of Horticultural Sciences, Agriculture Research, Education and Extension Organization (AREEO), Islamic Republic of Iran.
3- Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran. ,
Abstract: (1583 Views)
To investigate the infection of carnations (Dianthus caryophillus) to common viruses, a total of 130 samples showing suspected viral disease symptoms were collected from 10 commercial greenhouses located in Mahallat City, Markazi Province. Total DNA and RNA were extracted and amplified by PCR and RT-PCR methods, respectively, using generic primers for the genera Potyvirus and Orthotospovirus and also specific primers of Carnation mottle virus (CarMV), Carnation necrotic fleck virus (CNFV), Carnation etched ring virus (CERV), Carnation latent virus (CLV), Carnation vein mottle virus (CVMV) and Cucumber mosaic virus (CMV). Results revealed that the estimated rates of infection in the collected samples for different viruses in decreasing order were for CarMV, CNFV, CERV, CMV and CVMV, while CLV and species of genus Orthotospovirus were not detected. Mixed viral infections were also investigated and among all of the 130 collected samples, 3.1% showed mixed infections with CarMV, CNFV, CVMV, CERV and CMV, 32% with CarMV, CNFV, and CERV, 20% with CarMV and CNFV, and 9% with CarMV and CERV. For phylogenetical assays, NIb-CP fragment of the CVMV genome was amplified, cloned, and sequenced. Phylogenetic trees of two isolates of this virus revealed that the two Iranian isolates of CVMV (IBR and RCM) were phylogenetically divergent. Mechanical inoculation of CVMV on 16 different indicator plant species showed specific symptoms on six of the indicators that were confirmed by RT-PCR. To the best of our knowledge, this is the first report of CMV and first partial genome sequencing of CVMV from a carnation plant in Iran.
Article Type: Original Research |
Subject:
Plant Protection/Plant virology
Received: 2019/09/8 | Accepted: 2021/03/20 | Published: 2021/04/25
Received: 2019/09/8 | Accepted: 2021/03/20 | Published: 2021/04/25
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