Efficiency of Green Synthesized Silver Nanoparticles with Sweet Orange, Citrus sinensis (L.) (Rutaceae, Sapindales) against Tribolium confusum Duval. (Coleoptera, Tenebrionidae)

Document Type : Original Research

Authors
A. Sedighi 1
S. Imani 1
G. R. Moshtaghi Kashanian 2
H. Najafi 3
Y. Fathipour 4
1 Department of Entomology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
2 Department of Biochemistry, Medical School, Kerman University of Medical Sciences, Kerman, Islamic Republic of Iran.
3 Department of Weed Research, Iranian Research Institute of Plant Protection, Tehran, Islamic Republic of Iran.
4 Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
Abstract
The post-harvest damage caused by several pests is a major problem in stored grain product industry. Recently, synthesizing NanoParticles (NPs) with plant extracts act as an alternative approach in reduction of this damage. An attempt was made to synthesize and test the mortality effect of green synthesized silver NanoParticles (AgNPs) with peels extract of sweet orange, Citrus sinensis (L.) (Rutaceae, Sapindales), against the adult tenebrionid beetle Tribolium confusum (Duval). The synthesized nanoparticles were characterized with UV-visible spectroscopy, X-ray Diffraction, and Transmission Electron Microscopy. Five bioassays were conducted by filter-paper residue and feeding methods with different concentrations of both green synthesizing and commercial AgNPs. Results showed that citrus extract by itself was not toxic against beetles, however, the green AgNPs showed 83-77% mortality in filter-paper residue and feeding methods. The impact of commercial AgNPs in both methods led to 75 and 40% mortality. The lowest LC50 and LT50 for green synthesized AgNPs were recorded (30.62 ppm and 9.92 day) for filter-paper residue tests. According to the results, the green silver nanoparticles, showed more efficiency than the commercial ones. This provides an evidence that green synthesized AgNPs have a great potential as an alternative option in stored products pest management.

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Journal of Agricultural Science and Technology
Volume 21, Issue 6
September and October 2019
Pages 1485-1494