Distribution of Virachola livia (Lepidoptera: Lycaenidae) Eggs and Influence of Conspecific Aggregation and Avoidance Behavior

Authors
Jimah Research Station, Ministry of Agriculture, Sultanate of Oman.
Abstract
The oviposition behaviour and distribution of the eggs of carpophagous Virachola livia Klug (Lepidoptera: Lycaenidae) was studied on pomegranate for six years. Taylor’s power law and Iwao’s mean patchiness regression, and common k proved the aggregated distribution of the eggs on pomegranate fruits. The butterflies tend to oviposit one egg per fruit, indicating the existence of conspecific egg avoidance behaviour. The study showed that the selection of oviposition sites was affected by the part of the fruit body and the cardinal direction of the tree canopy. Results suggest that the oviposition behavior of V. livia and, consequently, the distribution of the eggs may be regulated by the conspecific aggregation and conspecific egg avoidance. It is apparent that the butterfly integrates these two behaviors to maximize the success of its reproduction.

Keywords


1. Atkinson, W. D. and Shorrocks, B. 1981. Competition on a Divided and Ephemeral Resource: A Simulation Model. J. Anim. Ecol., 50: 461-471.
2. Awadallah, A. M. 1966. The Biology and Control of the Pomegranate Fruit Butterfly Virachola livia (Klug) (Ord.: Lepidoptera, Fam. Lycaenidae). Doctoral Thesis. Univ. Cairo, Cairo, Egypt.
3. Bancroft, J. S. 2005. Dispersal and Abundance of Lygus hesperus in Field Crop. Environ. Ent., 34: 1517-1523.
4. Bernays, E. A. 1999. When Host Choice is a Problem for a Generalist Herbivore: Experiments with the Whitefly Bemisia tabaci. Ecologic. Entomol., 24: 260-267.
5. Cochran, G. A. 1977. Sampling Techniques. 3rd Edition, John Wiley and Sons, New York, 428 PP.
6. Darbemamieh, M., Fathipour, Y. and Kamali, K. 2011. Population Abundance and Seasonal Activity of Zetzellia pourmirzai (Acari: Stigmaeidae) and Its Preys Cenopalpus irani and Bryobia rubrioculus (Acari: Tetranychidae) in Sprayed Apple Orchards of Kermanshah, Iran. J. Agr. Sci. Tech., 13: 143-154.
7. Doak, P., Kareiva, P. and Kingsolver, J. 2006. Fitness Consequences of Choosy Oviposition for a Time-limited Butterfly. Ecol., 87: 395-408.
8. Doane, D. and Seward, L. 2010. Applied Statistics in Business and Economics. 3rd Edition, Mcgraw-Hill.
9. Duarte, M. and Robbins, R. K. 2009. Immature Stages of Calycopis bellera (Hewitson) and C. janeirica (Felder) (Lepidoptera, Lycaenidae, Theclinae, Eumaeini): Taxonomic Significance and New Evidence for Detritivory. Zootaxa, 2325: 39–61.
10. Elliot, J. M. 1977. Some Methods for the Statistical Analysis of Samples of Benthic Invertebrates. 2nd Edition, Freshwater Biological Association, Cambridge, United Kingdom.
11. Feng, M. G. and Nowierski, R. M. 1992. Variation in Spatial Patterns of the Russian Wheat Aphid (Homoptera: Aphididae) among Small Grains in the North Western United States. Environ. Entomol., 21: 1029-1034.
12. Heed, W. B. 1968. Ecology of Hawaiian Drosophilidae. Univ. Texas Publ., 818: 387-419.
13. Janz, N. 2002. Evolutionary Ecology of Oviposition Strategies. In: “Chemoecology of Insect Eggs and Egg Deposition”, (Eds.): Hilker, M. and Meiners, T.. Blackwell Publishing, Berlin, PP. 349-376.
14. Kellogg, T. A. 1985. Egg Dispersion Patterns and Egg Avoidance Behaviour in the Butterfly Pieris sisymbrii Bdv. (Pieridae). J. Lepid. Soc., 39: 268-275.
15. Marchand, D. and Mcneil, J. N. 2004. Avoidance of Intraspecific Competition via. Host Modification in a Grazing, Fruit-eating Insect. Anim. Behav., 67: 397-402.
16. Moradi-Vajargah, M., Golizadeh, A., Rafiee Dastjerdi, H., Zalucki, M. P., Hassanpour, M. and Naseri, B. 2011. Population Density and Spatial Distribution Pattern of Hypera postica (Coleoptera: Curculionidae) in Ardabil, Iran. Not. Bot. Horti. Agrobo., 39: 42-48. Available in http://www.notulaebotanicae.ro.pdf. [15 May 2014].
17. Rabasa, S. G., Gutierrez, D. and Escudero, A. 2005. Egg Laying by a Butterfly on a Fragmented Host Plant: A Multi-level Approach. Ecography, 28: 629-639.
18. Raitanena, J., Forsmana, J. T., Kiveläb, S. M., Mäenpääa, M. I. and Välimäkia, P. 2014. Attraction to Conspecific Eggs May Guide Oviposition Site Selection in a Solitary Insect. Behav. Ecol., 25: 110-116.
19. Rausher, M. D. 1979. Larval Habitat Suitability and Oviposition Preference in Three Related Butterflies. Ecol., 60: 503-511.
20. Rosenheim, J. A. 1999. The Relative Contributions of Time and Eggs to the Cost of Reproduction. Evol., 53(2): 376–385.
21. Schäpers A., Carlsson, M. A., Gamberale-Stille, G. and Janz, N. 2015. The Role of Olfactory Cues for the Search Behavior of a Specialist and Generalist Butterfly. J. Insect Behav., 28:77–87.
22. Schowalter, T. D. 2006. Insect Ecology: An Ecosystem Approach. Academic Press, London, 572 PP.
23. Scoble, M. J. 1995. The Lepidoptera: Form, Function and Diversity. Oxford University Press, Oxford, 404 PP.
24. Sedaratian, A., Fathipour, Y., Talebi, A. A. and Farahani, S. 2010. Population Density and Spatial Distribution Pattern of Thrips tabaci (Thysanoptera: Thripidae) on Different Soybean Varieties. J. Agr. Sci. Tech., 12: 275-288.
25. Shapiro, A. M. 1980. Egg-load Assessment and Carryover Diapause in Anthocaris (Pieridae). J. Lepid. Soc., 4: 307-315.
26. Southwood, T. R. E. 1978. Ecological Methods. The English Language Book, Society and Chapman and Hall University Printing House, Cambridge, Great Britain, 524 PP.
27. Sparks, T. H., Porter, K., Greatorex-Davies, J. N., Hall, M. L. and Marrs, R. H. 1994. The Choice of Oviposition Sites in Woodland by the Duke of Burgundy Butterfly Hamearis lucina in England. Biol. Conserv., 70: 257-264.
28. Storey-Palma, J., Benítez, H. A., Mundaca, E. A. and Vargas, H. A. 2014. Egg Laying Site Selection by a Host Plant Specialist Leaf Miner Moth at Two Intra-plant Levels in the Northern Chilean Atacama Desert. Revista Brasileira de Entomologia, 58(3): 280–284.
29. Takahashi, K. H., Tuno, N. and Kagaya, T. 2005. The Relative Importance of Spatial Aggregation and Resource Partitioning on the Coexistence of Mycophagous Insects. Oikos, 109: 125-134.
30. Taylor, L. R., Woiwod, I. P. and Perry, J. N. 1978. The Density-dependence of Spatial Behaviour and the Variety of Randomness. J. Anim. Ecol., 47: 383-406.
31. Thompson, J. N. 1988. Evolutionary Ecology of the Relationship between Oviposition Preference and Performance of Offspring in Phytophagous Insects. Entomologia Experimentalis et Applicata, 47: 3-14.
32. Thompson, J. N. and Pellmyr, O. 1991. Evolution of Oviposition Behaviour and Host Preference in Lepidoptera. Annu. Rev. Entomol., 36: 65-89.
33. Tsai, J. H., Wang, J. J. and Liu, Y. H. 2000. Sampling of Diaphorina citri (Homoptera: Psyllidae) on Orange Jessamine in Southern Florida. Florida Entomologist, 83: 446-459.
34. Wetzstein, H. Y., Zhang, Z., Ravid, N. and Wetzstein, M. E. 2011. Characterization of Attributes Related to Fruit Size in Pomegranate. HortSci., 46(6): 908–912.
35. Zar, J. H. 1999. Biostatistical Analysis. Prentice Hall International Editions, New Jersey, 929 PP.
36. Zonneveld, C., Longcore, T. and Mulder, C. 2003. Optimal Schemes to Detect the Presence of Insect Species. Conserv. Biol., 17: 476–487.