Volume 9, Issue 1 (2007)                   JAST 2007, 9(1): 61-68 | Back to browse issues page

‎ 20.1001.1.16807073.2007.9.1.6.1

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Nandakumar R, Babu S, Raguchander T, Samiyappan R. Chitinolytic Activity of Native Pseudomonas fluorescens Strains. JAST 2007; 9 (1) :61-68
URL: http://jast.modares.ac.ir/article-23-9261-en.html
Chitinolytic Activity of Native Pseudomonas fluorescens Strains
R. Nandakumar 1, S. Babu1 , T. Raguchander2 , R. Samiyappan1
1- Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 640 003, India.
2- Sugarcane Research Station, Semmandalam, Cuddalore 607 001, Tamil Nadu, India.
Abstract:   (5498 Views)
The chitinase producing ability of Pseudomonas fluorescens strains viz., PF1, PB2 and FP7 was evaluated in a culture medium with and without a chitin source. The addition of 1% (v/v) chitin in culture medium significantly increased the bacterial population and chitinase activity. Among three strains tested, FP7 responded well to the addition of chitin by producing 31.2% increased chitinase in culture. Western blot analysis with chitinase antibody detected six and five chitinase isoforms in culture inoculated with FP7 and PF1, respectively.
Keywords: Biological control, Chitin, Chitinase, Pseudomonas fluorescens
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Received: 2010/01/30 | Accepted: 2010/01/30 | Published: 2010/01/30
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