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Analysis of matrix metalloproteinase-9 promoter region activity and association analysis of promoter region SNPs with lactation traits in dairy goats
Qing Li1 , Tianle Chao1 , Rong Xuan1 , Yunpeng Chu1 , Shujun Wang1 , Ming Cheng2 , Peipei Li2 , Zhibin Ji1 , Jianmin Wang 3
1- College of Animal Science and Veterinary Medicine, Shandong Agricultural University
2- Qingdao Animal Husbandry and Veterinary Research Institution
3- College of Animal Science and Veterinary Medicine, Shandong Agricultural University , wangjm@sdau.edu.cn
Abstract:   (60 Views)
Matrix metalloproteinase-9 (MMP9) degrades the extracellular matrix (ECM), participates in mammary gland remodeling, and inhibits mammary epithelial cell apoptosis in goats. To investigate the transcriptional regulatory mechanism of the MMP9 promoter region, we analyzed the expression pattern of MMP9 in dairy goats by qRT-PCR and cloned the promoter region by PCR. Deletion analysis indicated that the MMP9 gene core promoter region was located upstream of the transcription start site in the -715 bp to -926 bp region. Identification of two Specificity protein 1 (Sp1) binding sites affecting the promoter activity of the MMP9 by targeted mutagenesis. The c.1863 G>A mutation in the MMP9 gene increased the promoter transcriptional activity and may be associated with an additional serum response factor (SRF) transcription factor-binding site. The association analysis revealed that c.1863 G>A was significantly associated with milk fat percentage in dairy goats, which was significantly higher in goats with the AG genotype (3.90 ± 0.04%) than in goats with the GG genotype (3.73 ± 0.05%). This study lays the foundation for subsequent analysis of the transcriptional regulatory mechanism of MMP9 and exploration of its biological functions
Keywords: goat, MMP9, promoter, Sp1, SNP
Full-Text [PDF 899 kb]   (47 Downloads)    
Article Type: Original Research | Subject: Animal Genetics
Received: 2022/11/25 | Accepted: 2023/06/11 | Published: 2024/03/31
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.