Volume 25, Issue 2 (2023)
JAST 2023, 25(2): 443-456 |
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Hatice Havutcu G, Aksoy M. Metallophosphoesterases of Chlamydomonas reinhardtii and Analyses of Their Transcription Levels Under Phosphate Deficiency. JAST 2023; 25 (2) :443-456
URL: http://jast.modares.ac.ir/article-23-57046-en.html
URL: http://jast.modares.ac.ir/article-23-57046-en.html
1- Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, Türkiye.
2- Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, Türkiye. , maksoy@akdeniz.edu.tr
2- Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, Türkiye. , maksoy@akdeniz.edu.tr
Abstract: (748 Views)
Phosphorous (P) is an important macroelement for all organisms. However, there is a finite amount of P on Earth, therefore, new enzymes and technologies are needed for better P use in agriculture. Metallophosphoesterases are a large group of evolutionarily related proteins that are important in biotechnology. We found fourteen putative Metallophosphoesterase (MPA) genes in the genome of Chlamydomonas reinhardtii. Our RT-PCR analyses showed that some of these genes were constitutively expressed, and some were upregulated under phosphate deficiency. These results and bioinformatic analyses suggest that two of the genes (MPA11 and MPA13) are transcribed in high levels and the putative polypeptides are predicted to be secreted to extracellular space, making them ideal to be used in biotechnological applications. Phylogenetic analyses show that MPA11 and MPA13 are related to known phytases from plant species, suggesting MPA11 and MPA13 might have specific phytase activity. In light of these results, we discuss the potential of C. reinhardtii as a phytase producing organism for agricultural and industrial use.
Article Type: Original Research |
Subject:
Genetic Engineering
Received: 2021/11/9 | Accepted: 2022/04/27 | Published: 2023/03/5
Received: 2021/11/9 | Accepted: 2022/04/27 | Published: 2023/03/5
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