Volume 25, Issue 2 (2023)                   JAST 2023, 25(2): 469-484 | Back to browse issues page

‎ 10.52547/jast.25.2.469

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Saberi F, Marzban R, Ardjmand M, Pajoum Shariati F, Tavakoli O. Optimization of the Culture Medium, Fermentation Process, and Effectiveness of a Biopesticide from an Iranian Bacillus thuringiensis var. tenebrionis (BN2). JAST 2023; 25 (2) :469-484
URL: http://jast.modares.ac.ir/article-23-55133-en.html
Optimization of the Culture Medium, Fermentation Process, and Effectiveness of a Biopesticide from an Iranian Bacillus thuringiensis var. tenebrionis (BN2)
F. Saberi1 , R. Marzban 2, M. Ardjmand3 , F. Pajoum Shariati1 , O. Tavakoli4
1- Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
2- Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Islamic Republic of Iran. , r.marzban@areeo.ac.ir
3- Department of Chemical Engineering, Tehran South Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
4- School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Islamic Republic of Iran.
Abstract:   (966 Views)
The local strain of B. thuringiensis var. tenebrionis-BN2 (Btt-BN2) was used to control the alfalfa weevil. Experimental design using Response Surface Methodology (RSM) was applied for the optimization of the culture medium and fermentation parameters in order to achieve a high level of Colony-Forming Unit (CFU) (spore mL−1). The parameters including the amount of carbon and nitrogen sources at three levels were investigated with CFU (spore mL−1) response for two sets of experiments. The obtained results showed that the maximum CFU (spore mL−1) for the minimum concentration of oat (2 g L-1), the minimum concentration of corn steep liquor (10 g L-1), and the maximum concentration of sugarcane molasses (10 g L-1) were equivalent to 1.4×1013 spore mL-1. Optimum fermentation parameters to obtain the highest value of CFU (spore mL−1) were determined as a maximum level of pH of eight and a medium level of temperature (280C). Amazingly, optimum conditions enhanced the CFU value to 8.06× 1013 spore mL-1, which is very significant in the Btt research. Finally, the bioassay analysis of Btt in a single system and binary system (combination of two insecticides; Matrine® and Abamectin®) at different concentrations illustrated 83% of mortality efficiency (3 ppm of Btt and 0.5 ppm Matrine) on the 3rd day of treatment and 100% efficiency almost for all combination of Btt with Matrine and/or Abamectin after the 7th day. The bioassay results showed promising environmentally friendly mortality efficiency compared to the current chemical treatments.
Keywords: Alfalfa weevil, Biological control, Fermentation parameters, Integrated Pest Management, Mortality.
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Article Type: Original Research | Subject: Agricultural Economics/Agriculture Marketing and Supply Chains
Received: 2021/08/26 | Accepted: 2022/04/13 | Published: 2023/03/5
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