Volume 2, Issue 1 (2000)
JAST 2000, 2(1): 33-39 |
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Mojdehi H, Singleton L L. Reaction of Wheat Varieties to Infection by Pythium
arrhenomanes or its Toxic Metabolite (s). JAST 2000; 2 (1) :33-39
URL: http://jast.modares.ac.ir/article-23-1786-en.html
URL: http://jast.modares.ac.ir/article-23-1786-en.html
1- Islamic Azad University, Tehran, Islamic Republic of Iran.
2- Departement of Plant Pathology, Oklahoma State University, Stillwater, Ok. 74078, U.S.A.
2- Departement of Plant Pathology, Oklahoma State University, Stillwater, Ok. 74078, U.S.A.
Abstract: (5492 Views)
Eight wheat varieties some of which were earlier reported to be resistant to Pythium
arrhennmanes, were inoculated with P. arrhenomanes. Inoculation was performed by
placing 2-day-old seedlings on the edge of a P. arrhenomanes culture on corn meal
agar for 3 h at 25°C. Infected seedlings were then transferred to test tubes (9 mm
dia.) containing glass beads and 1 ml of sterile water, and incubated for 4 days at
25°O Non-inoculated seedlings were exposed to the toxic metabolite(s) from P.
arrhenomanes by placing them in test tubes containing 1 ml of a 25-day-old culture
filtrate of P. arrhenomanes. Non-inoculated seedlings grown in test tubes containing
sterile water served as controls. Measurements were taken on root and shoot length,
as well as fresh and dry root and shoot weights. There was an indication that it may
be possible to use the toxic metabolite(s) in screening for resistance to P.
arrhenomanes. Although all of the varieties appeared to be susceptible, there were
some differences among them in their reaction to fungal infection or exposure to the
toxic metabolite(s).
Received: 2010/03/15 | Accepted: 2010/03/15 | Published: 2010/03/15
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