Volume 21, Issue 1 (2019)                   JAST 2019, 21(1): 221-232 | Back to browse issues page

‎ 20.1001.1.16807073.2019.21.1.1.0

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Levic J, Munaut F, Scauflaire J, Stankovic S, Ivanovic D, Krnjaja V. Polyphasic Approach Used for Distinguishing Fusarium temperatum from Fusarium subglutinans. JAST 2019; 21 (1) :221-232
URL: http://jast.modares.ac.ir/article-23-12766-en.html
Polyphasic Approach Used for Distinguishing Fusarium temperatum from Fusarium subglutinans
J. Levic1 , F. Munaut2 , J. Scauflaire2 , S. Stankovic 1, D. Ivanovic1 , V. Krnjaja3
1- Maize Research Institute, Zemun Polje, Belgrade-Zemun, Belgrade, Republic of Serbia.
2- Earth and Life Institute, Laboratory of Mycology, Mycology of the Catholic University of Louvain, Catholic University of Louvain, Belgium.
3- Institute for Animal Husbandry, Belgrade-Zemun, Republic of Serbia.
Abstract:   (6822 Views)
Morphological, biological, and phylogenetic approaches were undertaken for the identification of pathogenic species F. temperatum in the Serbian population of F. subglutinans collected in the 1999-2010 period from Zea mays (3 root, 15 stalk, and 6 seed samples), Sorghum bicolor (two seed samples), Hordeum vulgare (one seed sample) and Taraxacum officinale (one seed sample). Based on interspecies mating compatibility analyses and the maximum parsimony analysis of EF-1α sequences, only two strains, originating from S. bicolor seed (MRIZP 0418 and MRIZP 0552), were identified as F. temperatum, while the remaining 26 single-spore strains were identified as F. subglutinans Group 2. In situ detached barley leaf assay and artificially stalk and ear inoculation of two maize hybrids demonstrated that both F. temperatum and F. subglutinans strains were medium and strong pathogens under laboratory and field conditions, respectively. These are the first data on the F. temperatum as seed-borne pathogens of sorghum, as well as pathogenicity of F. temperatum strains on maize.
 
Keywords: Maize, Maximum parsimony analysis, Polyphasic approach of identification, Sorghum seed.
Full-Text [PDF 728 kb]   (2078 Downloads)    
Article Type: Research Paper | Subject: Agricultural Economics/Agriculture Marketing and Supply Chains
Received: 2017/03/17 | Accepted: 2018/04/21 | Published: 2018/12/30
Add your comments about this article : Your username or Email:
CAPTCHA
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.